Choosing a SynBase™ Solid Support

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LINK’s SynBase™ CPG solid supports are available in a variety of pore sizes and functionalised nucleoside loadings. Four pore sizes are offered from 500Å to 3000Å. The 500Å/110 support comes in standard (35-50μmol/g) and high (60-100μmol/g) loading varieties (product names are appended with either an ‘S’ or ‘H’ respectively where there is a choice), with also a high-load 500Å/50 version. The range has been designed to offer optimum performance depending on the application, and the required nucleoside loading and length of sequence. The range is summarised below.

Shorter primer molecules (ca. 20 bases) can be synthesised on the 500Å support. Largescale synthesis (typically of oligos 40 bases or less in length) is most economical using the higher loading. Medium-length DNA (20-80 bases) for the widest range of applications, is best synthesised using the 1000Å support. Most companies in truth use 1000Å for all small scale synthesis (40nmol - 10μmol) regardless of oligo length. Indeed, this support is the one we routinely use when preparing all 3’-modifier supports.

For very long sequences (>80 bases) we recommend either the 2000Å or 3000Å support, the latter performing better >120 bases. 3000Å CPG is also available functionalised as a Phosphate (2398) and Spacer C3 (2395) CPG. Other 3’-modifier CPGs prepared with 2000/3000Å support may be available as custom products so please contact us with your requirements.

 

Features Table

Product Average Pore Size (Å) Nominal Particle Size (µm) Nucleoside Loading (µmol/g)
SynBase™ CPG 500/50 H

500

50

80-130

SynBase™ CPG 500/110 S

500

110

35-50

SynBase™ CPG 500/110 H

500

110

60-100

SynBase™ CPG 1000/110

1000

110

25-40

SynBase™ CPG 2000/110

2000

110

15-35

SynBase™ CPG 3000/110

3000

110

10-25

 

Optimum Use Table

 

<20 Bases

20-80
Bases

80-120
Bases

>80 Bases

Larger-scale
Synthesis

SynBase™ CPG
500/50 H

     

SynBase™ CPG
500/110 S

       

SynBase™ CPG
500/110 H

     

SynBase™ CPG
1000/110

     

SynBase™ CPG
2000/110

       

SynBase™ CPG
3000/110

     

 

 

Applies to products 2255, 2256, 2257, 2258, 2261, 2262, 2263, 2264, 2265, 2266, 2267, 2268, 2271, 2272, 2273, 2274, 2275, 2277, 2278, 2317, 2331, 2332, 2333, 2334, 2335, 2336, 2357, 2360, 2375, 2376, 2377, 2378, 2381, 2382, 2383, 2384, 2386, 2395, 2398.

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