New Potential Oligonucleotide Delivery Reagents

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Poster presented at the OTS Annual Meeting (2013) in Naples.

Abstract

Labelling oligonucleotides with lypophilic compounds such as cholesterol as a means of improving cell penetration is well known. Link offers a range of oligonucleotide synthesis reagents for use in this field and the cholesterol and tocopherol modifiers have proved particularly successful. Subsequently we have developed new reagents we believe have the potential to aid the delivery of oligonucleotides into a cell. These are currently being tested for their ability to deliver different oligonucleotide types into various cell lines.

Here we describe the use of one example of the new reagents in terms of use in oligonucleotide synthesis and the results of evaluating a range of siRNA molecules modified with all current reagents available from Link along with five new modifications in terms of their ability to target the PTENpg1 asRNA alpha variant. PTEN (gene) is a tumour suppressor gene phosphatase and tensin hololog. This regulates the cell cycle and mutations of this gene have been identified in a large number of cancers making this an important gene in terms of cancer therapy. This has been shown to be under the regulatory control of the PTEN pseudogene expressed noncoding RNA, PTENpg1.

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