A Comparison of Hydrophilic and Hydrophobic Amino and Thiol Linkers for use in Post-Synthetic Labelling of Oligonucleotides

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Poster presented at IRT 2010 - XIX International Round Table on Nucleosides, Nucleotides and Nucleic Acids, Lyon, France.

Abstract

Thiol and amino linkers are widely used as a means of post-synthetically labelling oligonucleotides. In general these are hydrophobic in nature but are often used in conjunction with hydrophilic spacers such as HEG (spacer 18) and spacer 9. There now exist hydrophilic thiol and amino linkers that alleviate the need for the additional hydrophilic spacer. The use of these in post synthetic labelling of oligonucleotides with HRP, fluorescein and thioctic acid has been evaluated.

Applicable to: 2123, 2124, 2125, 2126, 2129, 2182, 2187 and 2193.

Related to: 2133, 2532 and 2534.

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