A Novel Bz-Thiol-dT Phosphoramidite for Internal Post-Synthetic Labelling of Oligonucleotides with Maleimide and Acetamide Functionalised Labels

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Poster presented at NACON VIII, Sheffield, UK (2010).

Abstract

Although thiol-modified oligonucleotides are routinely used to introduce labels such as dyes, haptens and enzymes via reaction with maleimides or acetamides, this method has been limited to the 5’ or 3’ end of the oligonucleotide. The use of thiol reactive labels for internal modification until now required the conversion of an amino functionality, e.g. amino dT with thioctic acid. We have developed a thiol dT modification that can be incorporated within an oligonucleotide sequence and react directly with maleimides and acetamides.

Applicable to: 2125, 2126, 2135, 2166, 2187 and 2191.

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