3'-Amino Modification

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Applicable Products

2350 3'-Amino-Modifier-C7 CPG 1000
2365 3'-PT-Amino-Modifier-C6 CPG
2367 3'-Amino-Modifier-C6-dT CPG
2369 3'-Amino-Modifier-C6-dC CPG
2371 3'-PT-Amino-Modifier-C3 CPG

Physical Data

Item

Unit Wt.

2350 209.18
2365 179.15
2367 359.24
2369 457.42
2371 137.07

Coupling

All supports are used as per unmodified nucleoside supports. However, non-nucleosidic modifications are slow to detritylate and require an initial detritylation prior to use in synthesis. In this case it is important not to use a cycle with an initial capping step. AMA deprotection will require dmf-G and Ac-C to be used.

Cleavage & Deprotection

2350—Cleavage of the oligonucleotide requires 2h at room temperature with ammonium hydroxide or AMA. It is important to wash the column with 10-20% diisopropylamine in acetonitrile or 10-20% DEA/acetonitrile to prevent blocking with acrylonitrile during deprotection. Alternatively, 20% piperidine in DMF can be used, rather than DEA. In fact this will simultaneously remove Fmoc and cyanoethyl protection, plus remove the vinylfluorene by-product from the Fmoc deprotection reaction.

For the PT-Amino CPGs (2371 & 2365)—Cleavage of the oligonucleotide requires overnight at 55°C with ammonium hydroxide. This treatment will complete the deprotection of the nucleobases for all protection types. It is important to wash the column with 10-20% diisopropylamine in acetonitrile or 10-20% DEA/acetonitrile to prevent blocking with acrylonitrile during deprotection.

2369 & 2367—Use deprotection conditions as required by the nucleobases. It is important to wash the column with 10-20% diisopropylamine in acetonitrile or 10-20% DEA/acetonitrile to prevent blocking with acrylonitrile during deprotection.

Storage & Stability

The supports are stored in a freezer at –10 to –30°C.

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