|2005||dT-H Phosphonate, TEA Salt|
|2006||dG-H Phosphonate, TEA Salt|
|2007||dA-H Phosphonate, TEA Salt|
|2035||dC-H Phosphonate, DBU Salt|
Please note that the liquid reagents used in H-phosphonate synthesis are unfortunately not available from Link Technologies.
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile/pyridine (1:1). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
All monomers must be dissolved in acetonitrile/ pyridine (1:1).
Coupling & Capping1
Normal deblock is used, however activation is carried out with 1-adamantane carbonyl chloride dissolved in acetonitrile/pyridine (95:5). Capping is carried out with the TEA salt of isopropyl phosphite.
There are two oxidation steps and they need only be carried out at the end of the synthesis. The first is accomplished using 0.1M iodine in pyridine/ N-methylimidazole/water/THF (5:1:5:89), and the second with 0.1M iodine in triethylamine/water/ THF (5:5:90).
Standard oligonucleotide deprotection conditions can be applied when deprotecting an oligo synthesised using these products.
Storage & Stability
Refrigerate monomers. Stability in solution is approximately 1 week.
- Novel activating and capping reagents for improved hydrogenphosphonate DNA synthesis, A. Andrus, J.W. Efcavitch, L.T. McBride and B. Giusti, Tetrahedron Lett., 29, 861-864, 1988.