Electrochemical Labelling (using Ferrocene-dT)

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Applicable Product

2167 Ferrocene-dT-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

2167 C60H73N6O10PFe 1125.09 684.46 2.22 4.44 8.89

Coupling

A 5min coupling time is recommended. Ac-dC and dmf-dG must be used in the oligo synthesis to allow AMA deprotection.

Cleavage & Deprotection

Cleave and deprotect using AMA for 10min at 65˚C, followed by immediate desalting with G25 to remove the deprotection solution.

Purification

Best results are obtained using ion-exchange HPLC. Note to avoid oxidation of the ferrocene ring system it is recommended all solvents and buffers are deoxygenated with argon.

Storage & Stability

Store in a freezer at -10 to -30˚C. Acetonitrile solutions must be used within 24h.

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