Structural Studies - Use of 2-Aminopurine and 4-Thio-dT

Follow

Applicable Products

2069 2-Aminopurine-CE Phosphoramidite
2070 4-Thio-dT-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

2069 C43H53N8O6P 808.93 313.21 3.09 6.18 12.36
2070 C43H52N5O7PS 813.95 320.26 3.07 6.14 12.29

Coupling

No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites.

Oxidation

0.02M Oxidiser is recommended for 2070.

Deprotection

Use standard deprotection conditions with 2069. For 2070 it is desirable to remove the cyanoethyl protecting group using 1M DBU in acetonitrile for 3h at room temperature, prior to cleavage and deprotection. The latter is achieved with 50mM NaSH in ammonium hydroxide solution for 24h at room temperature.

Storage & Stability

Refrigerate dry compounds. Stability in solution is 2-3 days.

 

Have more questions? Submit a request

Comments

Powered by Zendesk