Reverse (5' to 3') Synthesis

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Applicable Products

2020 dT-5'-CE Phosphoramidite
2021 iBu-dG-5'-CE Phosphoramidite
2022 Bz-dA-5'-CE Phosphoramidite
2023 Bz-dC-5'-CE Phosphoramidite
2093 dmf-dG-5'-CE Phosphoramidite
2294 dT-5'-SynBase™ CPG 1000/110
2298 iBu-dG-5'-SynBase™ CPG 1000/110
2355 iBu-dG-5'-SynBase™ CPG 1000/110
2356 Bz-dC-5'-SynBase™ CPG 1000/110

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

2020 C40H49N4O8P 744.83 304.20 3.36 6.71 13.43
2021 C44H54N7O8P 839.94 329.21 2.98 5.95 11.91
2022 C47H52N7O7P 857.95 313.21 2.91 5.83 11.66
2023 C46H52N5O8P 833.93 289.18 3.00 6.00 11.99
2093 C43H53N8O7P 824.92 329.21 3.03 6.06 12.12
2294 - - 304.20 - - -
2298 - - 329.21 - - -
2355 - - 313.21 - - -
2356 - - 289.18 - - -

Coupling

No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites and supports.

Cleavage & Deprotection

Standard oligonucleotide deprotection conditions can be applied when deprotecting an oligo synthesised using these products.

Storage & Stability

All phosphoramidites are stored refrigerated at a maximum of 2-8°C. Their stability in solution is the same as standard dA, dC, dG and dT phosphoramidites. CPGs are stored in the fridge or freezer.

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