RNA Synthesis using 2’-OMe Chemistry

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Applicable Products

2041 2'-OMe-Bz-A-CE Phosphoramidite
2042 2'-OMe-Bz-C-CE Phosphoramidite
2043 2'-OMe-Ac-C-CE Phosphoramidite
2044 2'-OMe-dmf-G-CE Phosphoramidite
2045 2'-OMe-U-CE Phosphoramidite
2083 2'-OMe-Pac-A-CE Phosphoramidite
2084 2'-OMe-iPr-Pac-G-CE Phosphoramidite
2310 2'-OMe-U-SynBase™ CPG 1000/110
2311 2'-OMe-dmf-G-SynBase™ CPG 1000/110
2312 2'-OMe-Bz-A-SynBase™ CPG 1000/110
2313 2'-OMe-Bz-C-SynBase™ CPG 1000/110
2314 2'-OMe-Ac-C-SynBase™ CPG 1000/110

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

2041 C48H54N7O8P 887.97 343.24 2.82 5.63 11.26
2042 C47H54N5O9P 863.95 319.21 2.89 5.79 11.57
2043 C42H52N5O9P 801.88 319.21 3.12 6.24 12.47
2044 C44H56N8O8P 855.95 359.24 2.92 5.84 11.68
2045 C40H49N4O9P 760.82 320.20 3.29 6.57 13.14
2083 C49H56N7O9P 917.99 343.24 2.72 5.45 10.89
2084 C52H62N7O10P 976.07 359.24 2.56 5.12 10.25
2310 - - 320.20 - - -
2311 - - 359.24 - - -
2312 - - 343.24 - - -
2313 - - 319.21 - - -
2314 - - 319.21 - - -

Coupling

To optimise efficiency a 6min coupling time is recommended for the phosphoramidites. Use of the supports does not require any change to the synthesis cycle.

Cleavage & Deprotection

Standard oligonucleotide deprotection conditions can be applied when deprotecting an oligo synthesised using these products.

Ac-C (2043) must be used when employing AMA deprotection (10min at 65°C). UltraMILD deprotection can be used with Pac-A (2083), iPr-Pac-G (2084) and 2043. Bz-C (2042 or 2313) cannot be used with AMA deprotections.

Storage & Stability

All products are stored refrigerated at 2 to 8˚C. Stability in solution is 3-5 days.

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