2'-Fluoro "RNA" Synthesis

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Applicable Products

2079 2'-F-Ac-dC-CE Phosphoramidite
2080 2'-F-dU-CE Phosphoramidite
2081 2'-F-Bz-A-CE Phosphoramidite
2082 2'-F-iBu-G-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

2079 C41H49N5O8FP 789.84 307.18 3.17 6.33 12.66
2080 C39H46N4O8FP 748.79 308.16 3.34 6.68 13.35
2081 C47H51N7O7FP 875.93 331.20 2.85 5.71 11.42
2082 C44H53N7O8FP 857.91 347.19 2.91 5.83 11.66

Coupling

A 3min coupling time is recommended.

Cleavage & Deprotection

Use AMA for 2h at room temperature. Do not heat the oligos. It is necessary to use Fast (or UltraFast deprotection amidites for all other nucleoside incorporations.

Purification

Standard cartridge or RP-HPLC is generally used as a means of purification.

Storage & Stability

The solids are be stored in the freezer at –10 to –30°C. The dissolved amidites are stable in solution for 2-3 days..

A licence may be required from Isis Pharmaceuticals, Inc. to incorporate 2'-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 5,670,633, 6,005,087, 6,531,584 and foreign equivalents.

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