Using Universal Supports

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Applicable Products

2300 Universal-Q SynBase™ CPG 500/110
2304 Universal SynBase™ CPG 1000/110
2410 Universal-Q SynBase™ CPG 1000/110 S
2411 Universal-Q SynBase™ CPG 1000/110 H
2458 Universal-Q Polystyrene

General

Rather than having predetermined bases or modifications on the solid support, the use of Universal Supports allows the addition of 3’- base or modification to the support. This is useful for many reasons. It allows preparation of plates (96, 384 etc) in an easy and efficient manner without the risk of the wrong solid support being added to the well. It also allows the incorporation of base modifications at the 3’-end of an oligo where no modified solid support is commercially available, e.g. 2’-F-dU.

Deblocking

An initial additional detritylation is recommended as this step is much slower on universal supports than on standard base supports. This prevents N-1 oligos from the 3’-end.

Coupling

No changes are required from the standard synthesis method recommended by the synthesiser manufacturer. This is therefore determined by the oligo type and any modification present.

Oxidation

2304 - No changes are required from the standard synthesis method recommended by the synthesiser manufacturer.
2300/2410/2411/2458 - The linker is stable to capping mixtures, but is slightly labile in oxidiser solution (8% cleavage overnight which is the equivalent of approximately 2000 synthesis cycles on an average program).

Cleavage & Deprotection

2304 - In order to completely remove the universal linker one of the following need to be used: (1) ammonium hydroxide solution, 17h at 80°C; (2) AMA, 5h at 80°C; or (3) AMA, overnight at 55°C.
2300/2410/2411 - Contrary to previously published mild methods, the best conditions we have found to completely remove the Q linker during deprotection are AMA at 70°C for 2.5h or AMA at 80°C for 2h. For this reason it is inadvisable to use this support with modifications that require mild, ultramild or room temperature deprotection. Compatibilty with RNA is therefore mixed. We would not recommend the use of the support with TBDMS chemistry.

Storage & Stability

Refrigerate at 2-8°C. Material is stable for several years.

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