DNP Labelling

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Applicable Products

2549 DNP-TEG-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (ml) are shown for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products are diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.

Item

Mol. Formula

Mol. Wt.

Unit Wt.

250mg

500mg

1g

2549 C48H64N5O13P 950.04 509.41 2.63 5.26 10.53

Coupling, Deprotection & Purification

A 12-15min coupling time is recommended. Most deprotection conditions are applicable. For optimal yield, oligos are prepared DMT ON, with removal of the DMT group after cleavage and deprotection. Otherwise, the 1,2-diol configuration enables cleavage of the DNP-TEG during deprotection. A pre-treatment of the column with 10% diethylamine in acetonitrile for 2min at room temperature (2x) to remove cyanoethyl protecting groups, then a rinse with acetonitrile, can minimise the unwanted DNP-TEG cleavage.

Storage & Stability

Store in a freezer at –10 to –30°C. Stable in solution for 2-3 days.

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